Mucosal Adjuvants Delivered by a Mucoadhesive Patch for Sublingual Administration of Subunit Vaccines
Résumé
Among mucosal administrations of vaccine, the sublingual route has already proven to be able to induce potent systemic and mucosal immune response. However, the absence of a simple and compliant delivery system, and the lack of robust mucosal adjuvants are still impeding the de-velopment of sublingual vaccines. Here we describe a mucoadhesive patch made of layer-by-layer assembly of polysaccharides, chitosan and hyaluronic acid. The mucoadhesive patch was covered by adjuvanted nanoparticles carrying viral proteins. We showed that the nanoparticles effectively cross the outer layers of the sublingual mucosa to reach the epithelium. Furthermore, the en-capsulated adjuvants 3M-052 and mifamurtide, targeting toll like receptor (TLR) 7/8 and nucleo-tide-binding oligomerization domain-2 (NOD2) respectively, are still fully active after encapsula-tion into nanoparticles and exhibit a cytokine/chemokine signature similar to the mucosal gold standard adjuvant, the cholera toxin. However, the particulate adjuvants induced more moderate levels of pro-inflammatory interleukin (IL)-6 and keratinocyte chemoattractant (KC), suggesting a controlled activation of the innate immune response.
Domaines
Biotechnologies
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Monge_Mucosal adjuvant by mucoadhesive patch_2022.pdf (1.93 Mo)
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