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Preprints, Working Papers, ... (Preprint) Year : 2022

How to make and trap a pseudo-vesicle with a micropipette

Abstract

We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of {\alpha}-Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.

Dates and versions

hal-03865909 , version 1 (22-11-2022)

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Pierre Tapie, Alexis M. Prevost, Lorraine Montel, Léa-Laetitia Pontani, Elie Wandersman. How to make and trap a pseudo-vesicle with a micropipette. 2022. ⟨hal-03865909⟩
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