Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue eLife Année : 2022

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking

Résumé

Migrating cells present a variety of paths, from random to highly directional ones. While random movement can be explained by basal intrinsic activity, persistent movement requires stable polarization. Here, we quantitatively address emergence of persistent migration in (hTERT)–immortalizedRPE1 (retinal pigment epithelial) cells over long timescales. By live cell imaging and dynamic micropatterning, we demonstrate that the Nucleus-Golgi axis aligns with direction of migration leading to efficient cell movement. We show that polarized trafficking is directed toward protrusions with a 20-min delay, and that migration becomes random after disrupting internal cell organization. Eventually, we prove that localized optogenetic Cdc42 activation orients the Nucleus-Golgi axis. Our work suggests that polarized trafficking stabilizes the protrusive activity of the cell, while protrusive activity orients this polarity axis, leading to persistent cell migration. Using a minimal physical model, we show that this feedback is sufficient to recapitulate the quantitative properties of cell migration in the timescale of hours.
Fichier principal
Vignette du fichier
elife-69229-v2.pdf (9.38 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03867087 , version 1 (23-11-2022)

Identifiants

Citer

Kotryna Vaidžiulytė, Anne-Sophie Macé, Aude Battistella, William Beng, Kristine Schauer, et al.. Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking. eLife, 2022, 11, ⟨10.7554/eLife.69229⟩. ⟨hal-03867087⟩
33 Consultations
15 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More