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Article Dans Une Revue Nature Communications Année : 2022

A synaptomic analysis reveals dopamine hub synapses in the mouse striatum

Fabrice Cordelières
Stéphane Claverol
Sabrina Lacomme
Mélina Petrel
Christelle Martin
  • Fonction : Auteur
Vincent Pitard
  • Fonction : Auteur
Thomas Biederer

Résumé

Abstract Dopamine transmission is involved in reward processing and motor control, and its impairment plays a central role in numerous neurological disorders. Despite its strong pathophysiological relevance, the molecular and structural organization of the dopaminergic synapse remains to be established. Here, we used targeted labelling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes. We provide the proteome of dopaminergic synapses with 57 proteins specifically enriched. Beyond canonical markers of dopamine neurotransmission such as dopamine biosynthetic enzymes and cognate receptors, we validated 6 proteins not previously described as enriched. Moreover, our data reveal the adhesion of dopaminergic synapses to glutamatergic, GABAergic or cholinergic synapses in structures we named “dopamine hub synapses”. At glutamatergic synapses, pre- and postsynaptic markers are significantly increased upon association with dopamine synapses. Dopamine hub synapses may thus support local dopaminergic signalling, complementing volume transmission thought to be the major mechanism by which monoamines modulate network activity.
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Dates et versions

hal-03876508 , version 1 (28-11-2022)

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Vincent Paget-Blanc, Marlene Pfeffer, Marie Pronot, Paul Lapios, Maria-Florencia Angelo, et al.. A synaptomic analysis reveals dopamine hub synapses in the mouse striatum. Nature Communications, 2022, 13 (1), pp.3102. ⟨10.1038/s41467-022-30776-9⟩. ⟨hal-03876508⟩
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