%0 Unpublished work %T Membrane-remodeling protein ESCRT-III homologs incarnate the evolution and morphogenesis of multicellular magnetotactic bacteria %+ Helmholtz-Zentrum Geesthacht (GKSS) %+ Ming Hsieh Department of Electrical Engineering [Los Angeles] %+ Research Center for Proteome Analysis Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences %+ Yale School of Medicine [New Haven, Connecticut] (YSM) %+ Centre d'Immunologie de Marseille - Luminy (CIML) %+ CAS Key Laboratory of Marine Ecology and Environmental Science (KLMEES) %+ Laboratoire de chimie bactérienne (LCB) %+ Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC) %+ International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms ( LIA-MagMC) %+ Xi'an Jiaotong University (Xjtu) %+ Endothélium, valvulopathies et insuffisance cardiaque (EnVI) %+ Architecture et fonction des macromolécules biologiques (AFMB) %+ CAS Institute of Oceanology (IOCAS) %A Zhang, Wenyan %A Chen, Jianwei %A Dai, Jie %A Zhu, Shiwei %A Guenno, Hugo Le %A Kosta, Artemis %A Pan, Hongmiao %A Qian, Xin-Xin %A Santini, Claire-Lise %A Menguy, Nicolas %A Li, Xuegong %A Chen, Yiran %A Liu, Jia %A Cui, Kaixuan %A Zhao, Yicong %A Liu, Guilin %A Durand, Eric %A Zhang, Wei-Jia %A Roussel, Alain %A Xiao, Tian %A Wu, Long-Fei %8 2022-12-12 %D 2022 %R 10.1101/2022.11.08.515611 %Z Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]Preprints, Working Papers, ... %X Abstract Endosomal sorting complex required transport (ESCRT) III proteins are essential for membrane remodeling and repair across all domains of life. Eukaryotic ESCRT-III and the cyanobacterial homologs PspA and Vipp1/Imm30 remodel membrane into vesicles, rings, filaments and tubular rods structures. Here our microscopy analysis showed that multicellular bacteria, referred to as magnetoglobules, possess multiple compartments including magnetosome organelles, polyphosphate granules, vesicles, rings, tubular rods, filaments and MVB-like structures. Therefore, membrane remodeling protein PspA might be required for the formation of these compartments, and contribute to the morphogenesis and evolution of multicellularity. To assess these hypotheses, we sequenced nine genomes of magnetoglobules and found a significant genome expansion compared to unicellular magnetotactic bacteria. Moreover, PspA was ubiquitous in magnetoglobules and formed a distinct clade on the tree of eubacterial and archaeal ESCRT-III. The phylogenetic feature suggested the evolution of magnetoglobules from a unicellular ancestor of deltaproteobacterium. Hetero-expression of ellipsoidal magnetoglobule pspA2 gene alone in Escherichia coli resulted in intracellular membrane aggregation. GFP fusion labeling revealed polar location of PspA2 in rod-shaped unicells and regular interval location in filamentous cells. Cryo-electron tomography analysis showed filament bundle, membrane sacculus, vesicles and MVB-like structure in the cells expressing PspA2. Moreover, electron-dense area with a similar distribution as GFP-PspA2 foci in filamentous cells changed the inward orientation of the septum, which might interfere with the cell division. Collectively, these results show the membrane remodeling function of magnetoglobule PspA proteins, which may contribute to morphogenesis and the evolution of multicellularity of magnetotactic bacteria. %G English %L hal-03894042 %U https://cnrs.hal.science/hal-03894042 %~ MNHN %~ CNRS %~ UNIV-AMU %~ IMPMC %~ ALLINSP %~ GIP-BE %~ COMUE-NORMANDIE %~ UNIROUEN %~ SORBONNE-UNIVERSITE %~ SORBONNE-UNIV %~ SU-SCIENCES %~ INRAE %~ SU-TI %~ AFMB %~ ALLIANCE-SU %~ MICA-UNITES