WiChR, a highly potassium-selective channelrhodopsin for low-light one- and two-photon inhibition of excitable cells - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Science Advances Année : 2022

WiChR, a highly potassium-selective channelrhodopsin for low-light one- and two-photon inhibition of excitable cells

Christiane Grimm
Linda Tillert
Marilù Casini
  • Fonction : Auteur
Dimitrii Tanese
Benoît Forget
Franziska Schneider-Warme
Valentina Emiliani

Résumé

The electric excitability of muscle, heart, and brain tissue relies on the precise interplay of Na + - and K + -selective ion channels. The involved ion fluxes are controlled in optogenetic studies using light-gated channelrhodopsins (ChRs). While non-selective cation-conducting ChRs are well established for excitation, K + -selective ChRs (KCRs) for efficient inhibition have only recently come into reach. Here, we report the molecular analysis of recently discovered KCRs from the stramenopile Hyphochytrium catenoides and identification of a novel type of hydrophobic K + selectivity filter. Next, we demonstrate that the KCR signature motif is conserved in related stramenopile ChRs. Among them, WiChR from Wobblia lunata features a so far unmatched preference for K + over Na + , stable photocurrents under continuous illumination, and a prolonged open-state lifetime. Showing high expression levels in cardiac myocytes and neurons, WiChR allows single- and two-photon inhibition at low irradiance and reduced tissue heating. Therefore, we recommend WiChR as the long-awaited efficient and versatile optogenetic inhibitor.
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Dates et versions

hal-03896916 , version 1 (13-12-2022)

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Johannes Vierock, Enrico Peter, Christiane Grimm, Andrey Rozenberg, I-Wen Chen, et al.. WiChR, a highly potassium-selective channelrhodopsin for low-light one- and two-photon inhibition of excitable cells. Science Advances , 2022, 8 (49), ⟨10.1126/sciadv.add7729⟩. ⟨hal-03896916⟩
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