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Communication Dans Un Congrès Année : 2022

Metabolomic response of biofilms exposed to cobalt in short-term experiments

Résumé

Metabolites are low molecular weight molecules produced by organisms upon cellular activities such as photosynthesis, homeostasis processes or detoxification. They can thus be representative of modifications of cellular metabolism induced by changes in ambient water quality of organisms, such as increase in metal concentrations, as well as metal stress. Up to now, most studies have focused on examining either metabolic responses in one aquatic organism at a time or one metabolite in microorganism community, such as biofilms. In the present study, we aimed to evaluate modification of biofilms metabolome upon short-term exposure to cobalt to assess it potential use of early-warning biomarkers of Co contamination in freshwater. To that end, mature biofilms were exposed for 7 days in outdoor microcosms (15 L) to increasing concentrations of Co (4 in total), from 10-7 mol.L-1 (5.9 g.L-1) to 10-5 mol.L-1 (589 g.L-1). The temperature, pH and dissolved metal concentrations were monitored every day in exposure media whereas concentrations of the major cations, anions and dissolved organic carbon were measured at the beginning of the experiment. Biofilm samples were collected after 1, 3 and 7 days of exposure and were examined for their Co bioaccumulation (total and intracellular), biomass, chlorophyll concentration, and metabolic content using an untargeted approach. Very good correlations were observed between the logarithm of the dissolved Co concentration and the intracellular Co content at each exposure time (R2adj: 0.91, 0.95, 0.97 at days 1, 3 and 7, respectively). Biomass was negatively impacted at the two highest Co concentrations with a 35% decrease at 10-5 mol.L-1 after 7 days of exposure. A decrease in chlorophyll content was also observed at each studied concentrations at the longer time of exposure. In total, 2117 metabolites were measured in biofilms, with 159 which were annotated. Among those annotated, 39 were dysregulated by Co. A decrease in flavonoid synthesis was observed whereas an increase in lipid production or its precursors (phosphatidylethanolamine, sphingosine and lysodiacylglyceryltrimethyl-homoserines) was measured. Current analyses focused on drawing dose-response curves studies based on annotated and unannotated metabolites in order to asses Co effects on biofilms.
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Dates et versions

hal-03960363 , version 1 (27-01-2023)

Identifiants

  • HAL Id : hal-03960363 , version 1

Citer

Simon Colas, B Marie, Mathieu Milhe-Poutingon, Patrick Baldoni-Andrey, Amiel Boullemant, et al.. Metabolomic response of biofilms exposed to cobalt in short-term experiments. SETAC North America 43rd annual meeting, SETAC, Nov 2022, Pittsburgh (Pennsylvania), France. ⟨hal-03960363⟩
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