A deep continental aquifer downhole sampler for microbiological studies - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Frontiers in Microbiology Année : 2023

A deep continental aquifer downhole sampler for microbiological studies

Résumé

To be effective, microbiological studies of deep aquifers must be free from surface microbial contaminants and from infrastructures allowing access to formation water (wellheads, well completions). Many microbiological studies are based on water samples obtained after rinsing a well without guaranteeing the absence of contaminants from the biofilm development in the pipes. The protocol described in this paper presents the adaptation, preparation, sterilization and deployment of a commercial downhole sampler (PDSshort, Leutert, Germany) for the microbiological studying of deep aquifers. The ATEX sampler (i.e., explosive atmospheres) can be deployed for geological gas storage (methane, hydrogen). To validate our procedure and confirm the need to use such a device, cell counting and bacterial taxonomic diversity based on high-throughput sequencing for different water samples taken at the wellhead or at depth using the downhole sampler were compared and discussed. The results show that even after extensive rinsing (7 bore volumes), the water collected at the wellhead was not free of microbial contaminants, as shown by beta-diversity analysis. The downhole sampler procedure was the only way to ensure the purity of the formation water samples from the microbiological point of view. In addition, the downhole sampler allowed the formation water and the autochthonous microbial community to be maintained at in situ pressure for laboratory analysis. The prevention of the contamination of the sample and the preservation of its representativeness are key to guaranteeing the best interpretations and understanding of the functioning of the deep biosphere.
Fichier principal
Vignette du fichier
fmicb-13-1012400.pdf (3.3 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03962449 , version 1 (30-01-2023)

Identifiants

Citer

Magali Ranchou-Peyruse, Marion Guignard, Perla G Haddad, Sylvain Robin, Fabrice Boesch, et al.. A deep continental aquifer downhole sampler for microbiological studies. Frontiers in Microbiology, 2023, 13, pp.1012400. ⟨10.3389/fmicb.2022.1012400⟩. ⟨hal-03962449⟩
40 Consultations
33 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More