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Article Dans Une Revue (Data Paper) Molecular Cell Année : 2003

Transcriptional Mutagenesis Induced by Uracil and 8-Oxoguanine in Escherichia coli

Résumé

Cells exposed to DNA damaging agents in their natural environment do not undergo continuous cycles of replication but are more frequently engaged in gene transcription. Luciferase gene expression analysis with DNA templates containing uracil or 8-oxoguanine, placed at a defined position, indicated that in nondividing Escherichia coli cells, efficient mutagenic lesion bypass does occur in vivo during transcription. Sequence analyses of the transcript population revealed that RNA polymerase inserts adenine opposite to uracil, and adenine or cytosine opposite to 8-oxoguanine. Surprisingly, deletions were also detected for 8-oxoguanine-containing templates, indicating RNA polymerase slippage over this lesion. Genetic analyses showed that, in E coli, 8-oxoguanine is subject to transcription-coupled repair. Consequently, DNA damages alter transcription fidelity in vivo, which may lead to the production of mutant proteins that have the potential to change the phenotype of nondividing cells.

Dates et versions

hal-03979135 , version 1 (08-02-2023)

Identifiants

Citer

Damien Brégeon, Zara Doddridge, Ho Jin You, Bernard Weiss, Paul Doetsch. Transcriptional Mutagenesis Induced by Uracil and 8-Oxoguanine in Escherichia coli. Molecular Cell, 2003, 12 (4), pp.959-970. ⟨10.1016/s1097-2765(03)00360-5⟩. ⟨hal-03979135⟩
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