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Article Dans Une Revue Biochimica et Biophysica Acta:Biomembranes Année : 2023

Studying membrane fusion using supported lipid bilayers on superparamagnetic beads

Résumé

The fusion between two lipid membranes is a ubiquitous mechanism in cell traffic and pathogens invasion. Yet it is not well understood how two distinct bilayers overcome the energy barriers towards fusion and reorganize themselves to form a unique continuous bilayer. The magnitudes and numbers of these energy barriers are themselves an open question. To tackle these issues, we developed a new tool that allows to control the forces applied between two supported lipid bilayers (SLBs) deposited on superparamagnetic beads. By applying a magnetic field, the beads self-organize along field lines in chains of beads and compress the two membranes on the contact zone. Using the diffusion of fluorescently labelled lipids from one bilayer to the other allows us to identify fusion of the bilayers in contact. We applied increasing forces on SLBs and increased the occurrence of fusion. This experimental system allows the simultaneous study of tens of facing bilayers in a single experiment and mitigate the stochasticity of the fusion process. It is thus a powerful tool to test the various parameters involved in the membrane fusion process.
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Dates et versions

hal-03984609 , version 1 (12-02-2023)

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Mathieu Letrou, Sophie Cribier, Nicolas Rodriguez, Julien Heuvingh. Studying membrane fusion using supported lipid bilayers on superparamagnetic beads. Biochimica et Biophysica Acta:Biomembranes, 2023, 1865 (1), pp.184070. ⟨10.1016/j.bbamem.2022.184070⟩. ⟨hal-03984609⟩
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