Apelin and the proopiomelanocortin system: a new regulatory pathway of hypothalamic α-MSH release - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue AJP - Endocrinology and Metabolism Année : 2011

Apelin and the proopiomelanocortin system: a new regulatory pathway of hypothalamic α-MSH release

Annabelle Reaux-Le Goazigo
  • Fonction : Auteur
Nadia de Mota
  • Fonction : Auteur
Lydie Jeandel
  • Fonction : Auteur
Nicolas Chartrel
  • Fonction : Auteur
Claude Knauf
  • Fonction : Auteur
Carine Raad
  • Fonction : Auteur
Philippe Valet
  • Fonction : Auteur
Catherine Llorens-Cortes
  • Fonction : Auteur

Résumé

Neuronal networks originating in the hypothalamic arcuate nucleus (Arc) play a fundamental role in controlling energy balance. In the Arc, neuropeptide Y (NPY)-producing neurons stimulate food intake, whereas neurons releasing the proopiomelanocortin (POMC)-derived peptide α-melanocyte-stimulating hormone (α-MSH) strongly decrease food intake. There is growing evidence to suggest that apelin and its receptor may play a role in the central control of food intake, and both are concentrated in the Arc. We investigated the presence of apelin and its receptor in Arc NPY- and POMC-containing neurons and the effects of apelin on α-MSH release in the hypothalamus. We showed, by immunofluorescence and confocal microscopy, that apelin-immunoreactive (IR) neuronal cell bodies were distributed throughout the rostrocaudal extent of the Arc and that apelin was strongly colocalized with POMC, but weakly colocalized with NPY. However, there were numerous NPY-IR nerve fibers close to the apelin-IR neuronal cell bodies. By combining in situ hybridization with immunohistochemistry, we demonstrated the presence of apelin receptor mRNA in Arc POMC neurons. Moreover, using a perifusion technique for hypothalamic explants, we demonstrated that apelin-17 (K17F) increased α-MSH release, suggesting that apelin released somato-dendritically or axonally from POMC neurons may stimulate α-MSH release in an autocrine manner. Consistent with these data, hypothalamic apelin levels were found to be higher in obese db/db mice and fa/fa Zucker rats than in wild-type animals. These findings support the hypothesis that central apelin is involved in regulating body weight and feeding behavior through the direct stimulation of α-MSH release.
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Dates et versions

hal-03990052 , version 1 (15-02-2023)

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Annabelle Reaux-Le Goazigo, Laurence Bodineau, Nadia de Mota, Lydie Jeandel, Nicolas Chartrel, et al.. Apelin and the proopiomelanocortin system: a new regulatory pathway of hypothalamic α-MSH release. AJP - Endocrinology and Metabolism, 2011, 301 (5), pp.E955-E966. ⟨10.1152/ajpendo.00090.2011⟩. ⟨hal-03990052⟩
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