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Pré-Publication, Document De Travail Année : 2021

Dynamic enhancer partitioning instructs activation of a growth regulator during exit from naïve pluripotency

Maxim V.C. Greenberg
Aurélie Teissandier
  • Fonction : Auteur
Marius Walter
Daan Noordermeer
Deborah Bourc’his

Résumé

SUMMARY During early mammalian development, the genome undergoes profound transitions in chromatin states, topological organization and recruitment of cis regulatory factors involved in transcriptional control. How these three layers of gene regulation interact is the matter of intense research. The Zdbf2 gene—which is involved in growth control—provides a valuable model to study this question: upon exit from naïve pluripotency and prior to tissue differentiation, it undergoes a switch in usage from a distal to a proximal promoter, along with a switch in chromatin states, from polycomb to DNA methylation occupancy. Using an embryonic stem cell (ESC) culture system to mimic this period, we show here that four enhancers contribute to the Zdbf2 promoter switch, concomitantly with dynamic changes in chromosome architecture. Indeed, CTCF plays a key role in partitioning the locus in ESCs, to facilitate enhancer contact with the distal Zdbf2 promoter only. Partition relieving enhances proximal Zdbf2 promoter activity, as observed during differentiation or with mutants that lack local CTCF-based partition. Importantly, we show that CTCF-based regulation occurs independently of the polycomb and DNA methylation pathways. Our study reveals the importance of multi-layered regulatory frameworks to ensure proper spatio-temporal activation of developmentally important genes.

Dates et versions

halshs-03437256 , version 1 (19-11-2021)

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Maxim V.C. Greenberg, Aurélie Teissandier, Marius Walter, Daan Noordermeer, Deborah Bourc’his. Dynamic enhancer partitioning instructs activation of a growth regulator during exit from naïve pluripotency. 2021. ⟨halshs-03437256⟩
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