Macrophage plasticity in experimental atherosclerosis. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2010

Macrophage plasticity in experimental atherosclerosis.

Résumé

As in human disease, macrophages (MØ) are central players in the development and progression of experimental atherosclerosis. In this study we have evaluated the phenotype of MØ associated with progression of atherosclerosis in the apolipoprotein E (ApoE) knockout (KO) mouse model.We found that bone marrow-derived MØ submitted to M1 and M2 polarization specifically expressed arginase (Arg) II and Arg I, respectively. This distinct arginase expression was used to evaluate the frequency and distribution of M1 and M2 MØ in cross-sections of atherosclerotic plaques of ApoE KO mice. Early lesions were infiltrated by Arg I(+) (M2) MØ. This type of MØ favored the proliferation of smooth muscle cells, in vitro. Arg II(+) (M1) MØ appeared and prevailed in lesions of aged ApoE KO mice and lesion progression was correlated with the dominance of M1 over the M2 MØ phenotype. In order to address whether the M2->M1 switch could be due to a phenotypic switch of the infiltrated cells, we performed in vitro repolarization experiments. We found that fully polarized MØ retained their plasticity since they could revert their phenotype. The analysis of the distribution of Arg I- and Arg II-expressing MØ also argued against a recent recruitment of M1 MØ in the lesion. The combined data therefore suggest that the M2->M1 switch observed in vivo is due to a conversion of cells already present in the lesion. Our study suggests that interventional tools able to revert the MØ infiltrate towards the M2 phenotype may exert an atheroprotective action.
Fichier principal
Vignette du fichier
Macro_plos_one_2010.pdf (2.72 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

inserm-00512376 , version 1 (30-08-2010)

Identifiants

Citer

Jamila Khallou-Laschet, Aditi Varthaman, Giulia Fornasa, Caroline Compain, Anh-Thu Gaston, et al.. Macrophage plasticity in experimental atherosclerosis.. PLoS ONE, 2010, 5 (1), pp.e8852. ⟨10.1371/journal.pone.0008852⟩. ⟨inserm-00512376⟩
195 Consultations
256 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More