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Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2014

One step synthesis of 6-oxo-cholestan-3β,5α-diol.

Résumé

Cholesterol metabolism has been recently linked to cancer, highlighting the importance of the characterization of new metabolic pathways in the sterol series. One of these pathways is centered on cholesterol-5,6-epoxides (5,6-ECs). 5,6-ECs can either generate dendrogenin A, a tumor suppressor present in healthy mammalian tissues, or the carcinogenic cholestane-3β,5α,6β-triol (CT) and its putative metabolite 6-oxo-cholestan-3β,5α-diol (OCDO) in tumor cells. We are currently investigating the identification of the enzyme involved in OCDO biosynthesis, which would be highly facilitated by the use of commercially unavailable [(14)C]-cholestane-3β,5α,6β-triol and [(14)C]-6-oxo-cholestan-3β,5α-diol. In the present study we report the one-step synthesis of [(14)C]-cholestane-3β,5α,6β-triol and [(14)C]-6-oxo-cholestan-3β,5α-diol by oxidation of [(14)C]-cholesterol with iodide metaperiodate (HIO4).
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Dates et versions

inserm-00968933 , version 1 (01-04-2014)

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Maud Voisin, Sandrine Silvente-Poirot, Marc Poirot. One step synthesis of 6-oxo-cholestan-3β,5α-diol.. Biochemical and Biophysical Research Communications, 2014, epub ahead of print. ⟨10.1016/j.bbrc.2014.01.138⟩. ⟨inserm-00968933⟩
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