NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Genome Biology Année : 2009

NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis

D. Vallenet
S. Floquet
  • Fonction : Auteur
  • PersonId : 919090
H. Ewles
  • Fonction : Auteur
  • PersonId : 1122697
C. Mouzé-Soulama
  • Fonction : Auteur
  • PersonId : 1122698
D. Brown
  • Fonction : Auteur
  • PersonId : 919091
A. Lajus
  • Fonction : Auteur
  • PersonId : 919092
C. Buchrieser
  • Fonction : Auteur
  • PersonId : 914003
C. Médigue

Résumé

BACKGROUND: Genome sequences, now available for most pathogens, hold promise for the rational design of new therapies. However, biological resources for genome-scale identification of gene function (notably genes involved in pathogenesis) and/or genes essential for cell viability, which are necessary to achieve this goal, are often sorely lacking. This holds true for Neisseria meningitidis, one of the most feared human bacterial pathogens that causes meningitis and septicemia. RESULTS: By determining and manually annotating the complete genome sequence of a serogroup C clinical isolate of N. meningitidis (strain 8013) and assembling a library of defined mutants in up to 60% of its non-essential genes, we have created NeMeSys, a biological resource for Neisseria meningitidis systematic functional analysis. To further enhance the versatility of this toolbox, we have manually (re)annotated eight publicly available Neisseria genome sequences and stored all these data in a publicly accessible online database. The potential of NeMeSys for narrowing the gap between sequence and function is illustrated in several ways, notably by performing a functional genomics analysis of the biogenesis of type IV pili, one of the most widespread virulence factors in bacteria, and by identifying through comparative genomics a complete biochemical pathway (for sulfur metabolism) that may potentially be important for nasopharyngeal colonization. CONCLUSIONS: By improving our capacity to understand gene function in an important human pathogen, NeMeSys is expected to contribute to the ongoing efforts aimed at understanding a prokaryotic cell comprehensively and eventually to the design of new therapies.
Fichier principal
Vignette du fichier
gb-2009-10-10-r110.pdf (824.57 Ko) Télécharger le fichier
GB-2009-10-10-R110-S1.EPS (1.84 Mo) Télécharger le fichier
GB-2009-10-10-R110-S2.XLS (165.5 Ko) Télécharger le fichier
GB-2009-10-10-R110-S3.XLS (391 Ko) Télécharger le fichier
GB-2009-10-10-R110-S4.XLS (52.5 Ko) Télécharger le fichier
gb-2009-10-10-r110.xml (187.14 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Format : Autre
Format : Autre
Format : Autre
Format : Autre
Format : Autre
Loading...

Dates et versions

pasteur-00663857 , version 1 (27-01-2012)

Identifiants

Citer

Christophe Rusniok, D. Vallenet, S. Floquet, H. Ewles, C. Mouzé-Soulama, et al.. NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis. Genome Biology, 2009, 10 (10), pp.R110. ⟨10.1186/gb-2009-10-10-r110⟩. ⟨pasteur-00663857⟩
736 Consultations
515 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More