%0 Journal Article %T Modification of phosphoinositides by the Shigella effector IpgD during host cell infection %+ Institut de Biologie Intégrative de la Cellule (I2BC) %+ Dynamique des Interactions Hôte-Pathogène - Dynamics of Host-Pathogen Interactions %A Tran van Nhieu, Guy %A Latour-Lambert, Patricia %A Enninga, Jost %Z JE and GTVN are funded by the Agence National pour la Recherche Projects “PureMagRupture” (JE, GTVN) and “RabReprogram” (JE). JE acknowledges support from the Institut Pasteur and the European Research Council (ERC-CoG “Endosubvert”). JE is member of the LabEx IBEID and Milieu Interieur. GTVN is supported by the Inserm (Institut National de la Santé et de la Recherche Médicale) and the CNRS (Centre National de la Recherche Scientifique) %< avec comité de lecture %@ 2235-2988 %J Frontiers in Cellular and Infection Microbiology %I Frontiers %V 12 %P 1012533 %8 2022-10-27 %D 2022 %R 10.3389/fcimb.2022.1012533 %M 36389142 %K Shigella %K IpgD %K phosphatidyl-inositol polyphosphate %K phosphatase %K invasion %K dissemination %K inflammation %K phosphotransferase %Z Life Sciences [q-bio]Journal articles %X Shigella, the causative agent of bacillary dysentery, subvert cytoskeletal and trafficking processes to invade and replicate in epithelial cells using an arsenal of bacterial effectors translocated through a type III secretion system. Here, we review the various roles of the type III effector IpgD, initially characterized as phosphatidylinositol 4,5 bisphosphate (PI4,5P 2 ) 4-phosphatase. By decreasing PI4,5P 2 levels, IpgD triggers the disassembly of cortical actin filaments required for bacterial invasion and cell migration. PI5P produced by IpgD further stimulates signaling pathways regulating cell survival, macropinosome formation, endosomal trafficking and dampening of immune responses. Recently, IpgD was also found to exhibit phosphotransferase activity leading to PI3,4P 2 synthesis adding a new flavor to this multipotent bacterial enzyme. The substrate of IpgD, PI4,5P 2 is also the main substrate hydrolyzed by endogenous phospholipases C to produce inositoltriphosphate (InsP 3 ), a major Ca 2+ second messenger. Hence, beyond the repertoire of effects associated with the direct diversion of phoshoinositides, IpgD indirectly down-regulates InsP 3 -mediated Ca 2+ release by limiting InsP 3 production. Furthermore, IpgD controls the intracellular lifestyle of Shigella promoting Rab8/11 -dependent recruitment of the exocyst at macropinosomes to remove damaged vacuolar membrane remnants and promote bacterial cytosolic escape. IpgD thus emerges as a key bacterial effector for the remodeling of host cell membranes. %G English %2 https://hal-pasteur.archives-ouvertes.fr/pasteur-03862984/document %2 https://hal-pasteur.archives-ouvertes.fr/pasteur-03862984/file/fcimb-12-1012533.pdf %L pasteur-03862984 %U https://hal-pasteur.archives-ouvertes.fr/pasteur-03862984 %~ PASTEUR %~ CEA %~ CNRS %~ OPENAIRE %~ CEA-UPSAY %~ I2BC %~ UNIV-PARIS-SACLAY %~ JOLIOT %~ CEA-DRF %~ UNIV-PARIS %~ UNIVERSITE-PARIS %~ UNIVERSITE-PARIS-SACLAY %~ ANR %~ GS-BIOSPHERA %~ GS-LIFE-SCIENCES-HEALTH %~ GS-HEALTH-DRUG-SCIENCES %~ TEST3-HALCNRS %~ DYNAMICS-HOST-PATHOGEN-INTERACTIONS %~ UMR3691