Deletion of the mitochondrial Pim1/Lon protease in yeast results in accelerated aging and impairment of the proteasome. - Biological Adaptation and Ageing Accéder directement au contenu
Article Dans Une Revue Free Radical Biology and Medicine Année : 2013

Deletion of the mitochondrial Pim1/Lon protease in yeast results in accelerated aging and impairment of the proteasome.

Résumé

The Saccharomyces cerevisiae homolog of the ATP-dependent Lon protease, Pim1p, is essential for mitochondrial protein quality control, DNA maintenance, and respiration. Here, we demonstrate that Pim1p activity declines in aging cells and that Pim1p deficiency shortens the replicative life span of yeast mother cells. This accelerated aging of pim1Δ cells is accompanied by elevated cytosolic levels of oxidized and aggregated proteins, as well as reduced proteasome activity. Overproduction of Hsp104p greatly diminishes aggregation of oxidized cytosolic proteins, rescues proteasome activity, and restores life span of pim1Δ cells to near wild-type levels. Our results show that defects in mitochondrial protein quality control have global intracellular effects leading to the increased generation of misfolded proteins and cytosolic protein aggregates, which are linked to a decline in replicative potential.

Dates et versions

hal-00787326 , version 1 (11-02-2013)

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Citer

Nika Erjavec, Aurélien Bayot, Monique Gareil, Nadine Camougrand, Thomas Nystrom, et al.. Deletion of the mitochondrial Pim1/Lon protease in yeast results in accelerated aging and impairment of the proteasome.. Free Radical Biology and Medicine, 2013, 56, pp.9-16. ⟨10.1016/j.freeradbiomed.2012.11.019⟩. ⟨hal-00787326⟩
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