Clearance of Genetic Variants of Amyloid β Peptide by Neuronal and Non-neuronal Cells - Biological Adaptation and Ageing Accéder directement au contenu
Article Dans Une Revue Protein & Peptide Letters Année : 2013

Clearance of Genetic Variants of Amyloid β Peptide by Neuronal and Non-neuronal Cells

Mai Panchal
  • Fonction : Auteur
Boutaina El Abida
  • Fonction : Auteur
Noureddine Lazar
  • Fonction : Auteur
Christine Fahy
  • Fonction : Auteur
Lionel Dubost
  • Fonction : Auteur
Mohamed Rholam
  • Fonction : Auteur

Résumé

The presence of senile plaques in the brain is one of the pathological hallmarks of Alzheimer’s disease (AD). The biogenesis and clearance of the amyloid β peptide (Aβ), the main component of the lesions, lie at the center of the pathogenesis of AD. In sporadic AD, the increase of Aβ levels seems to be indicative of failure of clearance mechanisms. We previously showed that the clearance of the wild type Aβ40 peptide by various neuronal and non-neuronal cells occurs through a same proteolytic process and that Aβ degradation was primarily dictated by its conformational state (Panchal et al., 2007). To gain further insights on the role of the peptide conformation in the clearance mechanism of Aβ, two Aβ40 peptides, known to be associated with amyloid angiopathy (Dutch and Flemish mutations), and the rodent Aβ40 peptide were catabolized by several cells by using the same experimental approach. The peptide fragments, generated by proteolytic cleavage of substrates in cell supernatants, were identified by LC-MS and the cleavage sites of proteases were deduced. In parallel, conformational states of wild type Aβ40 peptide and of the three Aβ40 variants were characterized by circular dichroism spectroscopy. We provide data suggesting that discrete conformational changes of Aβ40 peptide regulate its clearance rate by neuronal and non-neuronal cells. - See more at: http://www.eurekaselect.com/108425/article#sthash.MMBlmlwa.dpuf
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Dates et versions

hal-01544067 , version 1 (21-06-2017)

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Citer

Mai Panchal, Boutaina El Abida, Noureddine Lazar, Christine Fahy, Lionel Dubost, et al.. Clearance of Genetic Variants of Amyloid β Peptide by Neuronal and Non-neuronal Cells. Protein & Peptide Letters, 2013, 20 (5), pp.550-561. ⟨10.2174/0929866511320050008⟩. ⟨hal-01544067⟩
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