An inventory of 44 qPCR assays using hydrolysis probes operating with a unique amplification condition for the detection and quantification of antibiotic resistance genes - Centre International de Recherche en Infectiologie Accéder directement au contenu
Article Dans Une Revue Diagnostic Microbiology and Infectious Disease Année : 2021

An inventory of 44 qPCR assays using hydrolysis probes operating with a unique amplification condition for the detection and quantification of antibiotic resistance genes

Résumé

Early antibiotic resistance determinants (ARDs) detection in humans or animals is crucial to counteract theirpropagation. The ARDs quantification is fundamental to understand the perturbation caused by disruptors,such as antibiotics, during therapies. Forty-three qPCRs on the most diffused ARDs and integrons amonghuman and animal Enterobacterales, and one on the 16S rDNA for bacteria quantification, were developed.The qPCRs, using hydrolysis probes, operated with a unique amplification condition and were tested analyticallyand diagnostically performing 435 reactions on five positive and negative controls for each qPCR. Diagnosticsensitivity and specificity were confirmed by PCR and genome sequencing of control isolates,demonstrating 100% performance for all qPCRs. An easy and rapid discrimination method for the epidemiologicallyrelevant blaCTX-Ms is provided. This large, noncommercial qPCRs inventory could serve for precisequantification of ARDs, but also as a rapid screening tool for surveillance purposes, providing the basis forfurther high-throughput developments.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
S0732889321000213.pdf (1.28 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

anses-04143985 , version 1 (22-07-2024)

Licence

Identifiants

Citer

Tony Rochegüe, Marisa Haenni, Géraldine Cazeau, Véronique Metayer, Jean-Yves Madec, et al.. An inventory of 44 qPCR assays using hydrolysis probes operating with a unique amplification condition for the detection and quantification of antibiotic resistance genes. Diagnostic Microbiology and Infectious Disease, 2021, 100 (3), pp.115328. ⟨10.1016/j.diagmicrobio.2021.115328⟩. ⟨anses-04143985⟩
7 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More