Exploring the Impact of Homocysteine Supplementation on Avian Embryonic Metabolism Through Mass Spectrometry-Based Metabolomics Analysis - Génétique, reproduction et développement Accéder directement au contenu
Poster De Conférence Année : 2024

Exploring the Impact of Homocysteine Supplementation on Avian Embryonic Metabolism Through Mass Spectrometry-Based Metabolomics Analysis

Résumé

Embryonic malformations are often attributed to environmental factors, including folate deficiency. Avian models can recapitulate human folate deficiencies by adding homocysteine into the egg. However, how this treatment impacts the metabolite levels during embryonic development remains poorly understood. This project aims to fill this gap by studying how the supplementation of homocysteine affects the metabolite composition across various matrices. We used our expertise in mass spectrometry-based metabolomics to develop a method for sample preparation and analysis on the different parts of the egg: yolk, white, and embryo, in order to study the impact of homocysteine supplementation on metabolite profiles during embryonic development. Samples were analyzed using HILIC technology on a Vanquish-QExactive+ coupling (Thermo) in ESI-NEG and ESI-POS, full scan mode and targeted reprocessing on 150 metabolites. Our preliminary results show satisfactory metabolic coverage, with 103 metabolites detected across the 3 matrices studied. The next quantitative study of 60 samples will show the impact of homocysteine supplementation at different doses, on the metabolism of these 3 matrices at different stages of embryonic development. It will allow us to determine the dynamics of metabolite levels in homocysteine-supplemented eggs across developmental time.
Fichier principal
Vignette du fichier
202406_Poster_ROCHER_RFMF_vf.pdf (1.92 Mo) Télécharger le fichier

Dates et versions

hal-04634441 , version 1 (04-07-2024)

Identifiants

  • HAL Id : hal-04634441 , version 1

Citer

Amandine Rocher, Nina Lager-lachaud, Romane Bertrand, Charlène Guillot, Floriant Bellvert. Exploring the Impact of Homocysteine Supplementation on Avian Embryonic Metabolism Through Mass Spectrometry-Based Metabolomics Analysis. 16èmes JS RFMF, Jun 2024, Saint-Malo, France. . ⟨hal-04634441⟩
1 Consultations
1 Téléchargements

Partager

Gmail Mastodon Facebook X LinkedIn More