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Article Dans Une Revue PLoS ONE Année : 2015

Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation.

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Rotavirus NSP3 is a translational surrogate of the PABP-poly(A) complex for rotavirus mRNAs. To further explore the effects of NSP3 and untranslated regions (UTRs) on rotavirus mRNAs translation, we used a quantitative in vivo assay with simultaneous cytoplasmic NSP3 expression (wild-type or deletion mutant) and electroporated rotavirus-like and standard synthetic mRNAs. This assay shows that the last four GACC nucleotides of viral mRNA are essential for efficient translation and that both the NSP3 eIF4G- and RNA-binding domains are required. We also show efficient translation of rotavirus-like mRNAs even with a 5'UTR as short as 5 nucleotides, while more than eleven nucleotides are required for the 3'UTR. Despite the weak requirement for a long 5'UTR, a good AUG environment remains a requirement for rotavirus mRNAs translation.
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hal-01344143 , version 1 (28-05-2020)

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Matthieu Gratia, Patrice Vende, Annie Charpilienne, Hilma Carolina Baron, Cécile Laroche, et al.. Challenging the Roles of NSP3 and Untranslated Regions in Rotavirus mRNA Translation.. PLoS ONE, 2015, 11 (1), pp.e0145998. ⟨10.1371/journal.pone.0145998⟩. ⟨hal-01344143⟩
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