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Article Dans Une Revue Scientific Reports Année : 2017

Quorum-quenching limits quorum-sensing exploitation by signal-negative invaders

Résumé

Some bacteria produce and perceive quorum-sensing (QS) signals that coordinate several behaviours, including the costly processes that are exoenzyme production and plasmid transfer. In the case of plasmid transfer, the emergence of QS signal-altered invaders and their policing are poorly documented. In Agrobacterium tumefaciens, the virulence Ti-plasmid encodes both synthesis and sensing of QS-signals, which promote its transfer from a donor to a recipient cell. Here, we reported that QS-altered A. tumefaciens mutants arose during experimental evolution. All showed improved growth compared to their ancestor. Genome sequencing revealed that, though some had lost the Ti-plasmid, most were defective for QS-signal synthesis and Ti-plasmid conjugation (traR mutations) and one exhibited a QS-signal exploitation behaviour, using signal produced by other cells to enhance its own Ti-plasmid transfer. We explored mechanisms that can limit this QS-hijacking. We showed that the A. tumefaciens capacity to inactivate QS-signals by expressing QS-degrading enzyme could attenuate dissemination of the QS signal-negative Ti-plasmids. This work shows that enzymatic QSdisruption whether encoded by the QS-producing Ti-plasmid itself, by a companion plasmid in the same donor cells, or by one in the recipient cells, in all cases can serve as a mechanism for controlling QS exploitation by QS signal-negative mutants.
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hal-01602633 , version 1 (25-05-2020)

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Mélanie Tannieres, Julien Lang, Claudie Barnier, Jacqui A. Shykoff, Denis Faure. Quorum-quenching limits quorum-sensing exploitation by signal-negative invaders. Scientific Reports, 2017, 6, pp.1-10. ⟨10.1038/srep40126⟩. ⟨hal-01602633⟩
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