Mad1 influences interphase nucleoplasm organization and chromatin regulation in Drosophila - I2BC Accéder directement au contenu
Article Dans Une Revue Open Biology Année : 2018

Mad1 influences interphase nucleoplasm organization and chromatin regulation in Drosophila

Natacha Raich
  • Fonction : Auteur
  • PersonId : 1057903
Roger Karess

Résumé

The Drosophila Mad1 spindle checkpoint protein helps organize several nucleoplasmic components, and flies lacking Mad1 present changes in gene expression reflecting altered chromatin conformation. In interphase, checkpoint protein Mad1 is usually described as localizing to the inner nuclear envelope by binding the nucleoporin Tpr, an interaction believed to contribute to proper mitotic regulation. Whether Mad1 has other nuclear interphase functions is unknown. We found in Drosophila that Mad1 is present in nuclei of both mitotic and postmitotic tissues. Three proteins implicated in various aspects of chromatin organization co-immunoprecipitated with Mad1 from fly embryos: Mtor/Tpr, the SUMO peptidase Ulp1 and Raf2, a subunit of a Polycomb-like complex. In primary spermatocytes, all four proteins coloca-lized in a previously undescribed chromatin-associated structure called here a MINT (Mad1-containing IntraNuclear Territory). MINT integrity required all four proteins. In mad1 mutant spermatocytes, the other proteins were no longer confined to chromatin domains but instead dispersed throughout the nucleoplasm. mad1 flies also presented phenotypes indicative of excessive chromatin of heterochromatic character during development of somatic tissues. Together these results suggest that Drosophila Mad1, by helping organize its interphase protein partners in the nucleoplasm, contributes to proper chromatin regulation.
Fichier principal
Vignette du fichier
Raich et al 2018 official.pdf (1.49 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02355446 , version 1 (08-11-2019)

Identifiants

Citer

Natacha Raich, Souhir Mahmoudi, Doruk Emre, Roger Karess. Mad1 influences interphase nucleoplasm organization and chromatin regulation in Drosophila. Open Biology, 2018, ⟨10.1098/rsob.180166⟩. ⟨hal-02355446⟩
23 Consultations
38 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More