Article Dans Une Revue Nature Communications Année : 2025

Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae

Ariel Talavera
Eric Le Cam
Ole Skovgaard
Hedvig Tamman

Résumé

The replication of the two chromosomes in the pathogenic bacterium Vibrio cholerae is coordinated by the binding of initiator protein RctB to a checkpoint sequence, crtS . Replication of crtS on the primary chromosome (Chr1) triggers replication of the secondary chromosome (Chr2), but the details are poorly understood. Here, we analyze RctB binding patterns in the V. cholerae genome across various cell cycle stages. We find that RctB primarily binds to sites inhibiting replication initiation at the Chr2 origin ( ori2 ). This inhibitory effect is counteracted when crtS is replicated on Chr1, causing a shift in RctB binding to sites that activate replication at ori2 . Structural analyzes indicate the formation of diverse oligomeric states of RctB, coupled to the allosteric effect of DNA, which determine ori2 accessibility. We propose a synchronization model where, upon replication, crtS locally destabilizes the RctB inhibition complex, releasing the Chr2 replication origin.
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hal-04932113 , version 1 (06-02-2025)

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Theophile Niault, Ariel Talavera, Eric Le Cam, Sonia Baconnais, Ole Skovgaard, et al.. Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae. Nature Communications, 2025, 16 (1), pp.485. ⟨10.1038/s41467-024-55598-9⟩. ⟨hal-04932113⟩
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