Unraveling human, rodent and snake<i>Kolmioviridae</i>replication to anticipate cross-species transmission - Institut de recherche sur les maladies virales et hépatiques Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

Unraveling human, rodent and snakeKolmioviridaereplication to anticipate cross-species transmission

Lena Soppa
Leonora Szirovicza
Cedric Leyrat

Résumé

The recent discovery of Hepatitis D (HDV)-like viruses across a wide range of taxa led to the establishment of the Kolmioviridae family. Recent studies suggest that kolmiovirids can be satellites of viruses other than Hepatitis B virus (HBV), challenging the strict HBV/HDV-association dogma. Studying whether kolmiovirids are able to replicate in any animal cell they enter is essential to assess their zoonotic potential. Here, we compared replication of three kolmiovirids: HDV, rodent (RDeV) and snake deltavirus (SDeV) in vitro and in vivo. We show that SDeV has the narrowest and RDeV the broadest host cell range. High resolution imaging of infected cells revealed nuclear viral hubs with a peculiar RNA-protein organization. Finally, in vivo hydrodynamic delivery of infectious clones showed that both HDV and RDeV, but not SDeV, efficiently replicate in mouse liver, forming massive nuclear viral hubs. Our comparative analysis lays the foundation for the discovery of specific host factors controlling Kolmioviridae host-shifting.

Domaines

Virologie
Fichier principal
Vignette du fichier
2023 Khalfi, Denis et al. biorxiv.pdf (15.39 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04209863 , version 1 (18-09-2023)

Identifiants

Citer

Pierre Khalfi, Zoé Denis, Giovanni Merolla, Carine Chavey, José Ursic-Bedoya, et al.. Unraveling human, rodent and snakeKolmioviridaereplication to anticipate cross-species transmission. 2023. ⟨hal-04209863⟩
63 Consultations
1 Téléchargements

Partager

Gmail Facebook X LinkedIn More