Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Developmental Cell Année : 2003

Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body.

Résumé

PtdIns(3,5)P(2) is required for cargo-selective sorting to the vacuolar lumen via the multivesicular body (MVB). Here we show that Ent3p, a yeast epsin N-terminal homology (ENTH) domain-containing protein, is a specific PtdIns(3,5)P(2) effector localized to endosomes. The ENTH domain of Ent3p is essential for its PtdIns(3,5)P(2) binding activity and for its membrane interaction in vitro and in vivo. Ent3p is required for protein sorting into the MVB but not for the internalization step of endocytosis. Ent3p is associated with clathrin and is necessary for normal actin cytoskeleton organization. Our results show that Ent3p is required for protein sorting into intralumenal vesicles of the MVB through PtdIns(3,5)P(2) binding via its ENTH domain.
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Dates et versions

hal-00153224 , version 1 (08-06-2007)

Identifiants

  • HAL Id : hal-00153224 , version 1
  • PUBMED : 12967568

Citer

Sylvie Friant, Eve Isabelle Pécheur, Anne Eugster, Fabrice Michel, Yaya Lefkir, et al.. Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body.. Developmental Cell, 2003, 5 (3), pp.499-511. ⟨hal-00153224⟩
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