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Article Dans Une Revue Radiology Année : 2008

Aortic aneurysms in a rat model: in vivo MR imaging of endovascular cell therapy.

Jean-François Deux
Jiangping Dai
  • Fonction : Auteur
Charlotte Rivière
Florence Gazeau
Jacky Roger
  • Fonction : Auteur
Jean-Noel Pons
  • Fonction : Auteur
Didier Letourneur
Frank P Boudghène
  • Fonction : Auteur
Eric Allaire
  • Fonction : Auteur

Résumé

PURPOSE: To prospectively evaluate in rats whether magnetic cell labeling can be used to noninvasively assess the technical success of endovascular cell therapy for abdominal aortic aneurysms (AAAs). MATERIALS AND METHODS: The study was approved by an institutional animal care and use committee. Vascular smooth muscle cells (VSMCs) labeled with iron oxide nanoparticles were seeded endovascularly in already formed AAAs. T2*-weighted gradient-echo and T2-weighted spin-echo magnetic resonance (MR) imaging was performed in vivo at 1.5 T before and 30 minutes after the injection of iron-loaded VSMCs (14 rats), nonlabeled VSMCs (three rats), or iron-free particles (three rats). Ten rats were euthanized shortly after the injection (day 0). Of the 10 remaining rats, which were seeded with iron-loaded cells, three were imaged on day 7 after cell delivery; three, on day 14; and four, on day 28; then they were euthanized. Ex vivo high-field-strength MR imaging of two AAAs was performed 28 days after cell delivery. Histologic examination of cross sections of all AAAs was performed. Statistical evaluations were performed with a nonparametric Wilcoxon correlation test. RESULTS: Magnetic cell labeling did not alter the capability of VSMCs to stabilize the diameter of the aneurysms. T2*-weighted gradient-echo images showed areas of hypointense signal within the aortic wall immediately and up to 1 month after cell therapy. The mean signal intensity decreased significantly after cell delivery (from 2362 +/- 244 [standard deviation] before to 434 +/- 275 after delivery, P < .001). Areas of hypointense signal and iron-loaded VSMCs were colocalized in the area of aortic wall reconstruction at both high-field-strength MR imaging and histologic analysis. CONCLUSION: MR imaging with magnetic cell labeling can be used to document endovascular cell delivery in already formed AAAs in rats.
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Dates et versions

hal-00265774 , version 1 (20-03-2008)

Identifiants

Citer

Jean-François Deux, Jiangping Dai, Charlotte Rivière, Florence Gazeau, Philippe Méric, et al.. Aortic aneurysms in a rat model: in vivo MR imaging of endovascular cell therapy.. Radiology, 2008, 246 (1), pp.185-92. ⟨10.1148/radiol.2461070032⟩. ⟨hal-00265774⟩
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