Transcription enhancer factor-1-dependent expression of the alpha-tropomyosin gene in the three muscle cell types. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2006

Transcription enhancer factor-1-dependent expression of the alpha-tropomyosin gene in the three muscle cell types.

Résumé

In vertebrates, the actin-binding proteins tropomyosins are encoded by four distinct genes that are expressed in a complex pattern during development and muscle differentiation. In this study, we have characterized the transcriptional machinery of the alpha-tropomyosin (alpha-Tm) gene in muscle cells. Promoter analysis revealed that a 284-bp proximal promoter region of the Xenopus laevis alpha-Tm gene is sufficient for maximal activity in the three muscle cell types. The transcriptional activity of this promoter in the three muscle cell types depends on both distinct and common cis-regulatory sequences. We have identified a 30-bp conserved sequence unique to all vertebrate alpha-Tm genes that contains an MCAT site that is critical for expression of the gene in all muscle cell types. This site can bind transcription enhancer factor-1 (TEF-1) present in muscle cells both in vitro and in vivo. In serum-deprived differentiated smooth muscle cells, TEF-1 was redistributed to the nucleus, and this correlated with increased activity of the alpha-Tm promoter. Overexpression of TEF-1 mRNA in Xenopus embryonic cells led to activation of both the endogenous alpha-Tm gene and the exogenous 284-bp promoter. Finally, we show that, in transgenic embryos and juveniles, an intact MCAT sequence is required for correct temporal and spatial expression of the 284-bp gene promoter. This study represents the first analysis of the transcriptional regulation of the alpha-Tm gene in vivo and highlights a common TEF-1-dependent regulatory mechanism necessary for expression of the gene in the three muscle lineages.

Dates et versions

hal-00289730 , version 1 (23-06-2008)

Identifiants

Citer

Stéphanie Pasquet, François Naye, Corinne Faucheux, Odile Bronchain, Albert Chesneau, et al.. Transcription enhancer factor-1-dependent expression of the alpha-tropomyosin gene in the three muscle cell types.. Journal of Biological Chemistry, 2006, 281 (45), pp.34406-20. ⟨10.1074/jbc.M602282200⟩. ⟨hal-00289730⟩
294 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More