A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II is regulated by B3 domain transcription factors during seed development in Arabidopsis. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Plant Physiology Année : 2009

A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II is regulated by B3 domain transcription factors during seed development in Arabidopsis.

Hannetz Roschzttardtz
Ignacia Fuentes
  • Fonction : Auteur
Marcos Vásquez
  • Fonction : Auteur
Claudia Corvalán
  • Fonction : Auteur
Gabriel León
  • Fonction : Auteur
Isabel Gómez
  • Fonction : Auteur
Loreto Holuigue
  • Fonction : Auteur
Jesús Vicente-Carbajosa
  • Fonction : Auteur
Xavier Jordana
  • Fonction : Auteur

Résumé

Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and the respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One of them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), is specifically expressed in the embryo during seed maturation, suggesting that SDH2-3 may have a role as the complex II iron-sulfur subunit during embryo maturation and/or germination. Here, we present data demonstrating that three abscisic acid-responsive elements and one RY-like enhancer element, present in the SDH2-3 promoter, are involved in embryo-specific SDH2-3 transcriptional regulation. Furthermore, we show that ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3), and LEAFY COTYLEDON2, three key B3 domain transcription factors involved in gene expression during seed maturation, control SDH2-3 expression. Whereas ABI3 and FUS3 interact with the RY element in the SDH2-3 promoter, the abscisic acid-responsive elements are shown to be a target for bZIP53, a member of the basic leucine zipper (bZIP) family of transcription factors. We show that group S1 bZIP53 protein binds the promoter as a heterodimer with group C bZIP10 or bZIP25. To the best of our knowledge, the SDH2-3 promoter is the first embryo-specific promoter characterized for a mitochondrial respiratory complex protein. Characterization of succinate dehydrogenase activity in embryos from two homozygous sdh2-3 mutant lines permits us to conclude that SDH2-3 is the major iron-sulfur subunit of mature embryo complex II. Finally, the absence of SDH2-3 in mutant seeds slows down their germination, pointing to a role of SDH2-3-containing complex II at an early step of germination.

Dates et versions

hal-00426336 , version 1 (24-10-2009)

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Citer

Hannetz Roschzttardtz, Ignacia Fuentes, Marcos Vásquez, Claudia Corvalán, Gabriel León, et al.. A nuclear gene encoding the iron-sulfur subunit of mitochondrial complex II is regulated by B3 domain transcription factors during seed development in Arabidopsis.. Plant Physiology, 2009, 150 (1), pp.84-95. ⟨10.1104/pp.109.136531⟩. ⟨hal-00426336⟩

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