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Article Dans Une Revue Journal of Microbiological Methods Année : 2011

Metagenomic comparison of direct and indirect soil DNA extraction approaches

Tom Delmont
Patrick Robe
  • Fonction : Auteur
Ian M. Clark
  • Fonction : Auteur
Pascal Simonet
Timothy M Vogel

Résumé

Full pyrosequencing runs of both direct-extracted (high yield, low DNA size) and indirect-extracted DNA (low yield, high DNA size) from the same prairie soil show that the sequence distribution of the majority of the metabolic functions and species detected were statistically similar. Although some microbial functions differed at the 95% confidence interval in bootstrap analyses, the overall functional diversity was the same.

Dates et versions

hal-00629399 , version 1 (05-10-2011)

Identifiants

Citer

Tom Delmont, Patrick Robe, Ian M. Clark, Pascal Simonet, Timothy M Vogel. Metagenomic comparison of direct and indirect soil DNA extraction approaches. Journal of Microbiological Methods, 2011, 86 (3), pp.397-400. ⟨10.1016/j.mimet.2011.06.013⟩. ⟨hal-00629399⟩
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