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Article Dans Une Revue Curr Protoc Cell Biol Année : 2010

Isolation of nucleoli.

Résumé

Nucleoli are now recognized as multi-functional nuclear domains involved in several fundamental cell processes such as ribosome biogenesis, regulation of the assembly of non-ribosomal ribonucleoprotein complexes, tRNA maturation, sequestration of protein, viral infection, and cellular ageing. Extensive proteomic analyses of these nucleolar domains after their purification have contributed to the description of their multiple biological functions. Because nucleoli are the largest and densest nuclear structures, they are easily amenable to purification from nuclei of cultured animal cells using the protocol described in this unit.
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Dates et versions

hal-00709427 , version 1 (18-06-2012)

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Sabine Hacot, Yohann Coute, Stéphane Belin, Marie Alexandra Albaret, Hichem Mertani, et al.. Isolation of nucleoli.. Curr Protoc Cell Biol, 2010, Chapter 3, pp.Unit3.36. ⟨10.1002/0471143030.cb0336s47⟩. ⟨hal-00709427⟩
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