Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Clinical Endocrinology and Metabolism Année : 2011

Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells.

Résumé

CONTEXT: Empirical evidence suggests that autocrine human GH (hGH) may possess a proliferative and oncogenic role in human mammary carcinoma. However, this concept is largely derived from studies using cultured human mammary carcinoma cell (HMCC) lines. OBJECTIVE: We investigated the expression and functionality of hGH and the hGH receptor in isolated cultures of primary HMCC. Design: Epithelial cell adhesion molecule-positive primary HMCC were isolated from surgical biopsies of patients with mammary carcinoma and cultured in vitro. Expression of hGH and hGH receptor was determined by RT-PCR, immunofluorescence microscopy, and ELISA. The proliferative response of the cultured primary HMCC to hGH stimulation or hGH inhibition with a hGH antagonist was determined. RESULTS: One hundred percent of cultured primary HMCC expressed the hGH receptor, and 52% expressed hGH at the mRNA level. hGH-positive primary HMCC produced hGH protein within the cell and secreted hGH to the media. Both hGH-negative and hGH-positive HMCC responded to hGH stimulation with large increases in cell number. hGH-positive HMCC responded to inhibition of hGH by a hGH antagonist with a decrease in cell number, whereas hGH-negative HMCC did not. CONCLUSION: Primary HMCC proliferate in response to hGH, and the proliferation of hGH-positive HMCC is inhibited by hGH antagonism. Inhibition of hGH in patients with mammary carcinoma may therefore limit tumor growth.

Domaines

Cancer

Dates et versions

hal-00788316 , version 1 (14-02-2013)

Identifiants

Citer

Jean Chiesa, Catherine Ferrer, Cécile Arnould, Cécile M Vouyovitch, Jean-Jacques Diaz, et al.. Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells.. Journal of Clinical Endocrinology and Metabolism, 2011, 96 (9), pp.E1418-26. ⟨10.1210/jc.2011-0473⟩. ⟨hal-00788316⟩
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