LdFlabarin, a new BAR domain membrane protein of Leishmania Flagellum. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2013

LdFlabarin, a new BAR domain membrane protein of Leishmania Flagellum.

Résumé

During the Leishmania life cycle, the flagellum undergoes successive assembly and disassembly of hundreds of proteins. Understanding these processes necessitates the study of individual components. Here, we investigated LdFlabarin, an uncharacterized L. donovani flagellar protein. The gene is conserved within the Leishmania genus and orthologous genes only exist in the Trypanosoma genus. LdFlabarin associates with the flagellar plasma membrane, extending from the base to the tip of the flagellum as a helicoidal structure. Site-directed mutagenesis, deletions and chimera constructs showed that LdFlabarin flagellar addressing necessitates three determinants: an N-terminal potential acylation site and a central BAR domain for membrane targeting and the C-terminal domain for flagellar specificity. In vitro, the protein spontaneously associates with liposomes, triggering tubule formation, which suggests a structural/morphogenetic function. LdFlabarin is the first characterized Leishmania BAR domain protein, and the first flagellum-specific BAR domain protein.

Dates et versions

hal-00940309 , version 1 (31-01-2014)

Identifiants

Citer

Michèle Lefebvre, Emmanuel Tetaud, Magali Thonnus, Bénédicte Salin, Fanny Boissier, et al.. LdFlabarin, a new BAR domain membrane protein of Leishmania Flagellum.. PLoS ONE, 2013, 8 (9), pp.e76380. ⟨10.1371/journal.pone.0076380⟩. ⟨hal-00940309⟩
294 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More