Canthin-6-one Displays Antiproliferative Activity and Causes Accumulation of Cancer Cells in the G2/M Phase. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Asian Natural Products Research Année : 2014

Canthin-6-one Displays Antiproliferative Activity and Causes Accumulation of Cancer Cells in the G2/M Phase.

Résumé

Canthinones are natural substances with a wide range of biological activities, including antipyretic, antiparasitic, and antimicrobial. Antiproliferative and/or cytotoxic effects of canthinones on cancer cells have also been described, although their mechanism of action remains ill defined. To gain better insight into this mechanism, the antiproliferative effect of a commercially available canthin-6-one (1) was examined dose-dependently on six cancer cell lines (human prostate, PC-3; human colon, HT-29; human lymphocyte, Jurkat; human cervix, HeLa; rat glioma, C6; and mouse embryonic fibroblasts, NIH-3T3). Cytotoxic effects of 1 were investigated on the same cancer cell lines by procaspase-3 cleavage and on normal human skin fibroblasts. Strong antiproliferative effects of the compound were observed in all cell lines, whereas cytotoxic effects were very dependent on cell type. A better definition of the mechanism of action of 1 was obtained on PC-3 cells, by showing that it decreases BrdU incorporation into DNA by 60% to 80% and mitotic spindle formation by 70% and that it causes a 2-fold accumulation of cells in the G2/M phase of the cell cycle. Together, the data suggest that the primary effect of canthin-6-one (1) is antiproliferative, possibly by interfering with the G2/M transition. Proapoptotic effects might result from this disturbance of the cell cycle.
Fichier non déposé

Dates et versions

hal-01088758 , version 1 (24-01-2024)

Identifiants

  • HAL Id : hal-01088758 , version 1
  • PUBMED : 25379743

Citer

Camille Dejos, Pierre Voisin, Marianne Bernard, Matthieu Régnacq, Thierry Bergès. Canthin-6-one Displays Antiproliferative Activity and Causes Accumulation of Cancer Cells in the G2/M Phase.. Journal of Asian Natural Products Research, 2014, pp.2481-7. ⟨hal-01088758⟩
64 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More