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Poster De Conférence Année : 2010

Inflamatory response to cold plasma in normal skin

Résumé

Aim Investigate the effect of dose of cold plasma on skin integrity. Background Cold plasma is an emerging novel therapeutic approach in medicine with a great potential in auto-immune skin disorders, cutaneous infections and tumors. Only limited data are available on the safety of such a therapy. Therefore we investigated the cutaneous response in mice exposed to cold plasma. Methods We used BL6 mice which were exposed to plasma DBD (dielectric barrier discharge) in air at 2 mm of the skin for increasing doses to define the threshold of safety. The skin was excised at 24h and analyzed for integrity, neutrophil recruitment and cytokine and chemokine production and histological analyses. Results A dose dependent reddening of the skin was observed with scales at the highest dose. The macroscopic changes correlated with increased neutrophil recruitment in the skin as assessed by myeloperoxidase (MPO) activity, microscopically neutrophil infiltration of the epidermis extending in the dermis and at the highest dose in the subcutaneous fat tissue. Further, microabcesses in the epidermis with necrosis were found at the highest exposure dose. The measurement of inflammatory cytokines and chemokines associated with the inflammatory response is ongoing. The effect of high dose plasma was comparable to a local intradermal injection of endotoxin in terms of neutrophils infiltration. We hypothesized that epidermal barrier was damaged by the plasma allowing the entrance of commensals and their products into skin resulting in TLR depending activation of the innate immunity and inflammation. To test this hypothesis, we have ongoing studies using TLR2-4 and IL-1R deficient mice, which may have attenuated inflammation. In conclusion, a safe dose of plasma needs to be defined which conserves the epidermal integrity and avoids an inflammatory response.
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Dates et versions

hal-01151825 , version 1 (13-05-2015)

Identifiants

  • HAL Id : hal-01151825 , version 1

Citer

Marc Vandamme, Louis Fauconnier, Bernhard Ryffel, Jean-Michel Pouvesle, Valérie Quesniaux. Inflamatory response to cold plasma in normal skin. ICPM3, Sep 2010, Greifswald, Germany. ⟨hal-01151825⟩
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