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Article Dans Une Revue Methods in Molecular Biology Année : 2014

In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.

Résumé

Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ(54)), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ(54)-dependent gene expression.
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Dates et versions

hal-01219689 , version 1 (23-10-2015)

Identifiants

  • HAL Id : hal-01219689 , version 1
  • PUBMED : 25665558

Citer

Martin Buck, Christoph Engl, Nicolas Joly, Goran Jovanovic, Milija Jovanovic, et al.. In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.. Methods in Molecular Biology, 2014, 1276, pp.53-79. ⟨hal-01219689⟩
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