β-Catenin Activation in Muscle Progenitor Cells Regulates Tissue Repair - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cell Reports Année : 2016

β-Catenin Activation in Muscle Progenitor Cells Regulates Tissue Repair

Résumé

Skeletal muscle regeneration relies on a pool of resident muscle stem cells called satellite cells (MuSCs). Following injury-induced destruction of the myofibers, quiescent MuSCs are activated and generate transient amplifying progenitors (myoblasts) that will fuse to form new myofibers. Here, we focus on the canonical Wnt signaling pathway and find that either conditional β-catenin disruption or activation in adult MuSCs results in perturbation of muscle regeneration. Using both in vivo and in vitro approaches, we observed that myoblasts lacking β-catenin show delayed differentiation, whereas myoblasts with constitutively active β-catenin undergo precocious growth arrest and differentiation. Transcriptome analysis further demonstrated that Wnt/β-catenin signaling interacts with multiple pathways and, more specifically, TGF-β signaling. Indeed, exogenous TGF-β2 stimulation restores the regenerative potential of muscles with targeted β-catenin disruption in MuSCs. We conclude that a precise level of β-catenin activity is essential for regulating the amplification and differentiation of MuSC descendants during adult myogenesis.
Fichier principal
Vignette du fichier
1-s2.0-S2211124716304351-main.pdf (5.87 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01312388 , version 1 (06-05-2016)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Anja Rudolf, Elija Schirwis, Lorenzo Giordani, Alice Parisi, Christoph Lepper, et al.. β-Catenin Activation in Muscle Progenitor Cells Regulates Tissue Repair. Cell Reports, 2016, 15 (6), pp.1277-1290. ⟨10.1016/j.celrep.2016.04.022⟩. ⟨hal-01312388⟩
646 Consultations
281 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More