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Article Dans Une Revue Cell Reports Année : 2016

Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function

Marc Dalod

Résumé

Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomicdata with existing mRNA data to derive robust cell-specific expression signatures withmore than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1 beta secretion in response to ATP.

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Immunologie
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Dates et versions

hal-01438161 , version 1 (06-11-2018)

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Kuntal Worah, Till S. M. Mathan, Thien Phong Vu Manh, Shivakumar Keerthikumar, Gerty Schreibelt, et al.. Proteomics of Human Dendritic Cell Subsets Reveals Subset-Specific Surface Markers and Differential Inflammasome Function. Cell Reports, 2016, 16 (11), pp.2953-2966. ⟨10.1016/j.celrep.2016.08.023⟩. ⟨hal-01438161⟩
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