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Article Dans Une Revue PLoS Pathogens Année : 2012

Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses

Joel T. Van Mierlo
  • Fonction : Auteur
Alfred W. Bronkhorst
  • Fonction : Auteur
Gijs J. Overheul
  • Fonction : Auteur
Sajna A. Sadanandan
  • Fonction : Auteur
Jens-Ola Ekstrom
  • Fonction : Auteur
Marco Heestermans
  • Fonction : Auteur
Dan Hultmark
  • Fonction : Auteur
Christophe Antoniewski
Ronald P. Van Rij
  • Fonction : Auteur

Résumé

RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense.

Dates et versions

hal-01537140 , version 1 (12-06-2017)

Identifiants

Citer

Joel T. Van Mierlo, Alfred W. Bronkhorst, Gijs J. Overheul, Sajna A. Sadanandan, Jens-Ola Ekstrom, et al.. Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses. PLoS Pathogens, 2012, 8 (8), pp.e1002872. ⟨10.1371/journal.ppat.1002872⟩. ⟨hal-01537140⟩
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