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Article Dans Une Revue Plant Physiology Année : 2017

Constitutively active Arabidopsis map kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein

Résumé

Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana. CA-MPK3 plants are dwarfed and display a massive derepression of defense genes associated with spontaneous cell death as well as the accumulation of reactive oxygen species, phytoalexins, and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3/sid2 and CA-MPK3/ ein2-50 lines, which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3-associated phenotypes, indicating that the constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3- and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense-response system.

Dates et versions

hal-01606210 , version 1 (02-10-2017)

Identifiants

Citer

Baptiste Genot, Julien Lang, Souha Berriri, Marie Garmier, Françoise Gilard, et al.. Constitutively active Arabidopsis map kinase 3 triggers defense responses involving salicylic acid and SUMM2 resistance protein. Plant Physiology, 2017, 174 (2), pp.1238-1249. ⟨10.1104/pp.17.00378⟩. ⟨hal-01606210⟩
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