Human Induced Pluripotent Stem (hiPS) Cells from Urine Samples: A Non-Integrative and Feeder-Free Reprogramming Strategy - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Current Protocols in Human Genetics Année : 2017

Human Induced Pluripotent Stem (hiPS) Cells from Urine Samples: A Non-Integrative and Feeder-Free Reprogramming Strategy

Fanny Wulkan
  • Fonction : Auteur
Thayane Crestani
Graça Rosas
  • Fonction : Auteur
Rafael Dariolli
  • Fonction : Auteur
Alexandre C. Pereira
  • Fonction : Auteur
Jose E. Krieger
  • Fonction : Auteur

Résumé

Human induced pluripotent stem (hiPS) cell technology has already revolutionized some aspects of fundamental and applied research such as study of disease mechanisms and pharmacology screening. The first clinical trial using hiPS cell-derived cells began in Japan, only 10 years after the publication of the proof-of concept article. In this exciting context, strategies to generate hiPS cells have evolved quickly, tending towards non-invasive protocols to sample somatic cells combined with "safer" reprogramming strategies. In this unit, we describe a protocol combining both of these advantages to generate hiPS cells with episomal plasmid transfection from urine samples of individuals carrying the desired genotype. Based on previous published works, this simplified protocol requires minimal equipment and reagents, and is suitable both for scientists familiar with the hiPS cells technology and neophytes. HiPS cells displaying classical features of pluripotency and suitable for all desired downstream applications are generated rapidly (\textless10 weeks) and with high efficiency. © 2017 by John Wiley & Sons, Inc.
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Dates et versions

hal-01833116 , version 1 (09-07-2018)

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Citer

Clara Steichen, Karim Si-Tayeb, Fanny Wulkan, Thayane Crestani, Graça Rosas, et al.. Human Induced Pluripotent Stem (hiPS) Cells from Urine Samples: A Non-Integrative and Feeder-Free Reprogramming Strategy. Current Protocols in Human Genetics, 2017, Equipe IV, 92, pp.21.7.1--21.7.22. ⟨10.1002/cphg.26⟩. ⟨hal-01833116⟩
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