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Article Dans Une Revue Molecular Ecology Resources Année : 2019

A high-quality chromosome-level genome assembly of a generalist herbivore, Trichoplusia ni

Wenbo Chen
  • Fonction : Auteur
Xiaowei Yang
  • Fonction : Auteur
Guillaume Tetreau
Xiaozhao Song
  • Fonction : Auteur
Cathy Coutu
  • Fonction : Auteur
Gary Blissard
  • Fonction : Auteur
Ping Wang
  • Fonction : Auteur
  • PersonId : 985666

Résumé

The cabbage looper, Trichoplusia ni, is a globally distributed highly polyphagous herbivore and an important agricultural pest. T. ni has evolved resistance to various chemical insecticides, and is one of the only two insect species that have evolved resistance to the biopesticide Bacillus thuringiensis (Bt) in agricultural systems and has been selected for resistance to baculovirus infections. We report a 333-Mb high-quality T. ni genome assembly, which has N50 lengths of scaffolds and contigs of 4.6 Mb and 140 Kb, respectively, and contains 14,384 protein-coding genes. High-density genetic maps were constructed to anchor 305 Mb (91.7%) of the assembly to 31 chromosomes. Comparative genomic analysis of T. ni with Bombyx mori showed enrichment of tandemly duplicated genes in T. ni in families involved in detoxification and digestion, consistent with the broad host range of T. ni. High levels of genome synteny were found between T. ni and other sequenced lepidopterans. However, genome synteny analysis of T. ni and the T. ni derived cell line High Five (Hi5) indicated extensive genome rearrangements in the cell line. These results provided the first genomic evidence revealing the high instability of chromosomes in lepidopteran cell lines known from karyotypic observations. The high-quality T. ni genome sequence provides a valuable resource for research in a broad range of areas including fundamental insect biology, insect-plant interactions and co-evolution, mechanisms and evolution of insect resistance to chemical and biological pesticides, and technology development for insect pest management.

Dates et versions

hal-01988478 , version 1 (21-01-2019)

Identifiants

Citer

Wenbo Chen, Xiaowei Yang, Guillaume Tetreau, Xiaozhao Song, Cathy Coutu, et al.. A high-quality chromosome-level genome assembly of a generalist herbivore, Trichoplusia ni. Molecular Ecology Resources, 2019, 19 (2), pp.485-496. ⟨10.1111/1755-0998.12966⟩. ⟨hal-01988478⟩
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