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Article Dans Une Revue Human Molecular Genetics Année : 1993

Rapid physical mapping of YAC inserts by random integration of I-Sce I sites.

Résumé

We have developed a novel strategy, based on the random insertion by homologous recombination of artificial I-Sce I sites within mammalian repetitive DNA sequences, which should greatly facilitate the high resolution physical mapping of large DNA fragments cloned in YAC. A set of transgenic yeast strains containing appropriately spaced I-Sce I sites within the YAC insert defines a series of nested physical intervals against which new genes, clones or DNA fragments can be mapped by simple hybridisation. Sequential hybridisation using such a series of nested YAC fragments as probes can also allow the rapid sorting of phage or cosmid libraries into contigs. This approach, which has been applied to a YAC containing a 460 kb insert from the mouse X chromosome, may also have applications for the restriction mapping of large genomic segments, mapping of exons and the search for homologous genes.

Dates et versions

hal-02088067 , version 1 (02-04-2019)

Identifiants

Citer

Laurence Colleaux, P Colleaux, C. Rougeulle, P. Avner, B. Dujon. Rapid physical mapping of YAC inserts by random integration of I-Sce I sites.. Human Molecular Genetics, 1993, 2 (3), pp.265-71. ⟨10.1093/hmg/2.3.265⟩. ⟨hal-02088067⟩
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