Mt ZR1, a PRAF protein, is involved in the development of roots and symbiotic root nodules in Medicago truncatula - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Plant, Cell and Environment Année : 2014

Mt ZR1, a PRAF protein, is involved in the development of roots and symbiotic root nodules in Medicago truncatula

Résumé

PRAF proteins are present in all plants, but their functions remain unclear. We investigated the role of one member of the PRAF family, MtZR1, on the development of roots and nitrogen-fixing nodules in Medicago truncatula. We found that MtZR1 was expressed in all M.truncatula organs. Spatiotemporal analysis showed that MtZR1 expression in M.truncatula roots was mostly limited to the root meristem and the vascular bundles of mature nodules. MtZR1 expression in root nodules was down-regulated in response to various abiotic stresses known to affect nitrogen fixation efficiency. The down-regulation of MtZR1 expression by RNA interference in transgenic roots decreased root growth and impaired nodule development and function. MtZR1 overexpression resulted in longer roots and significant changes to nodule development. Our data thus indicate that MtZR1 is involved in the development of roots and nodules. To our knowledge, this work provides the first in vivo experimental evidence of a biological role for a typical PRAF protein in plants.
Fichier non déposé

Dates et versions

hal-02138587 , version 1 (23-05-2019)

Identifiants

Citer

Julie Hopkins, Olivier Pierre, Théophile Kazmierczak, Véronique Gruber, Florian Frugier, et al.. Mt ZR1, a PRAF protein, is involved in the development of roots and symbiotic root nodules in Medicago truncatula. Plant, Cell and Environment, 2014, 37 (3), pp.658-669. ⟨10.1111/pce.12185⟩. ⟨hal-02138587⟩
56 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More