Mitochondrial Transcriptome Control and Intercompartment Cross-Talk During Plant Development - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cells Année : 2019

Mitochondrial Transcriptome Control and Intercompartment Cross-Talk During Plant Development

Résumé

We address here organellar genetic regulation and intercompartment genome coordination. We developed earlier a strategy relying on a tRNA-like shuttle to mediate import of nuclear transgene-encoded custom RNAs into mitochondria in plants. In the present work, we used this strategy to drive trans-cleaving hammerhead ribozymes into the organelles, to knock down specific mitochondrial RNAs and analyze the regulatory impact. In a similar approach, the tRNA mimic was used to import into mitochondria in Arabidopsis thaliana the orf77, an RNA associated with cytoplasmic male sterility in maize and possessing sequence identities with the atp9 mitochondrial RNA. In both cases, inducible expression of the transgenes allowed to characterise early regulation and signaling responses triggered by these respective manipulations of the organellar transcriptome. The results imply that the mitochondrial transcriptome is tightly controlled by a “buffering” mechanism at the early and intermediate stages of plant development, a control that is released at later stages. On the other hand, high throughput analyses showed that knocking down a specific mitochondrial mRNA triggered a retrograde signaling and an anterograde nuclear transcriptome response involving a series of transcription factor genes and small RNAs. Our results strongly support transcriptome coordination mechanisms within the organelles and between the organelles and the nucleus
Fichier principal
Vignette du fichier
islandora_79538.pdf (3.86 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02184302 , version 1 (15-07-2019)

Licence

Paternité

Identifiants

Citer

Adnan Khan Niazi, E. Delannoy, Rana Khalid Iqbal, Daria Mileshina, R. Val, et al.. Mitochondrial Transcriptome Control and Intercompartment Cross-Talk During Plant Development. Cells, 2019, 8 (6), pp.583. ⟨10.3390/cells8060583⟩. ⟨hal-02184302⟩
51 Consultations
39 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More