Sequence requirements for premature transcription arrest within the first intron of the mouse c-fos gene. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Biology Année : 1991

Sequence requirements for premature transcription arrest within the first intron of the mouse c-fos gene.

Résumé

A strong block to the elongation of nascent RNA transcripts by RNA polymerase II occurs in the 5' part of the mammalian c-fos proto-oncogene. In addition to the control of initiation, this mechanism contributes to transcriptional regulation of the gene. In vitro transcription experiments using nuclear extracts and purified transcription templates allowed us to map a unique arrest site within the mouse first intron 385 nucleotides downstream from the promoter. This position is in keeping with that estimated from nuclear run-on assays performed with short DNA probes and thus suggests that it corresponds to the actual block in vivo. Moreover, we have shown that neither the c-fos promoter nor upstream sequences are absolute requirements for an efficient transcription arrest both in vivo and in vitro. Finally, we have characterized a 103-nucleotide-long intron 1 motif comprising the arrest site and sufficient for obtaining the block in a cell-free transcription assay.

Dates et versions

hal-02361089 , version 1 (13-11-2019)

Identifiants

Citer

Nadir Mechti, Marc Piechaczyk, J Blanchard, P. Jeanteur, B. Lebleu. Sequence requirements for premature transcription arrest within the first intron of the mouse c-fos gene.. Molecular and Cellular Biology, 1991, 11 (5), pp.2832-2841. ⟨10.1128/mcb.11.5.2832⟩. ⟨hal-02361089⟩
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