The lncRNA MARS modulates the epigenetic reprogramming of the marneral cluster in response to ABA - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2020

The lncRNA MARS modulates the epigenetic reprogramming of the marneral cluster in response to ABA

Résumé

Clustered organization of biosynthetic non-homologous genes is emerging as a characteristic feature of plant genomes. The co-regulation of clustered genes seems to largely depend on epigenetic reprogramming and three-dimensional chromatin conformation. Here we identified the long noncoding RNA (lncRNA) MARneral Silencing ( MARS ), localized inside the Arabidopsis marneral cluster, and which controls the local epigenetic activation of its surrounding region in response to ABA. MARS modulates the POLYCOMB REPRESSIVE COMPLEX 1 (PRC1) component LIKE-HETEROCHROMATIN PROTEIN 1 (LHP1) binding throughout the cluster in a dose-dependent manner, determining H3K27me3 deposition and chromatin condensation. In response to ABA, MARS decoys LHP1 away from the cluster and promotes the formation of a chromatin loop bringing together the MARNERAL SYNTHASE 1 ( MRN1 ) locus and a distal ABA-responsive enhancer. The enrichment of co-regulated lncRNAs in clustered metabolic genes suggests that the acquisition of noncoding transcriptional units constitute an additional regulatory layer driving the evolution of biosynthetic pathways.
Fichier principal
Vignette du fichier
2020.08.10.236562v2.full.pdf (1.83 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03016338 , version 1 (20-11-2020)

Identifiants

Citer

Thomas Roulé, Federico Ariel, Caroline Hartmann, Jose Gutierrez-Marcos, Nosheen Hussain, et al.. The lncRNA MARS modulates the epigenetic reprogramming of the marneral cluster in response to ABA. 2020. ⟨hal-03016338⟩
121 Consultations
355 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More