Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2015

Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation

Elmostafa Bahraoui
  • Fonction : Auteur
  • PersonId : 1073017

Résumé

Fibroblast growth factor 1 (FGF1) is induced during myoblast differentiation at both transcriptional and translational levels. Here, we identify hnRNPM and p54 nrb /NONO present in protein complexes bound to the FGF1 promoter and to the mRNA internal ribosome entry site (IRES). Knockdown or overexpression of these proteins indicate that they cooperate in activating IRES-dependent translation during myoblast differentiation, in a promoter-dependent manner. Importantly, mRNA transfection and promoter deletion experiments clearly demonstrate the impact of the FGF1 promoter on the activation of IRES-dependent translation via p54 nrb and hnRNPM. Accordingly, knockdown of either p54 or hnRNPM also blocks endogenous FGF1 induction and myotube formation, demonstrating the physiological relevance of this mechanism and the role of these two proteins in myogenesis. Our study demonstrates the cooperative function of hnRNPM and p54 nrb as regulators of IRES-dependent translation and indicates the involvement of a promoter-dependent mechanism.
Fichier principal
Vignette du fichier
pone.0136466.pdf (5.44 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03121844 , version 1 (19-03-2021)

Identifiants

Citer

Nadera Ainaoui, Fransky Hantelys, Edith Renaud-Gabardos, Morgane Bunel, Frédéric Lopez, et al.. Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation. PLoS ONE, 2015, 10 (9), pp.e0136466. ⟨10.1371/journal.pone.0136466⟩. ⟨hal-03121844⟩
28 Consultations
13 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More