LOW LEVEL OF BASELINE RESISTANCE IN RECENTLY HCV INFECTED MEN WHO HAVE SEX WITH MEN WITH HIGH-RISK BEHAVIORS - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Global Antimicrobial Resistance Année : 2021

LOW LEVEL OF BASELINE RESISTANCE IN RECENTLY HCV INFECTED MEN WHO HAVE SEX WITH MEN WITH HIGH-RISK BEHAVIORS

Résumé

Background: The presence of baseline HCV resistance-associated substitutions (RASs) can impair treatment outcome by Direct-Acting Antivirals. We investigated the prevalence of pretreatment HCV resistance among recently HCV infected MSM with high risk behaviors, either HIV co-infected or at high risk of HIV acquisition and under Pre-exposure Prophylaxis (PrEP). Methods: NS5A and NS3 fragments were deep sequenced on pretreatment samples of 72 subjects using Illumina Miseq paired-end sequencing technologies. UDS data was analyzed by Smartgene® platform. RASs mentioned in literature were analyzed and interpreted depending on genotypes (GT) at 10% cut-off. Results: HCV genotyping showed 36 (50%) GT1a, 31 (43.1%) GT4d, and 5 (6.9%) GT3a infections. Fifty-five (76.4%) patients were co-infected with HIV and 15 (20.8%) received PrEP. In GT1a virus, NS3-RASs were found in 4/30 viruses (13.3 %: S122 G/N, R155 K, and I170 V) and Q80 K polymorphism was present in 14/30 (46.7%) viruses. No NS3-RASs were detected in GT4d and 3a viruses. NS5A-RASs were detected in 3/36 GT1a viruses (8.3%: Q30E/R, L31 M, and H58 L). NS5A subtype-specific polymorphisms L30R and T58 P were found at high frequencies in 31/31 (100%) and 16/31 (51.6%) GT4d viruses, respectively. One RAS M31 L was also observed along with the polymorphisms L30R and T58 P. No NS5A-RASs were detected in GT3a viruses. Conclusions: A low level of RASs to NS3 and NS5A inhibitors on pretreatment samples was detected in the study population. Our findings reassure the clinical management of HCV infection in this high-risk population.
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Dates et versions

hal-03134798 , version 1 (08-02-2021)

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Thuy Nguyen, Marc-Antoine Valantin, Constance Delaugerre, Corinne Amiel, Emmanuelle Netzer, et al.. LOW LEVEL OF BASELINE RESISTANCE IN RECENTLY HCV INFECTED MEN WHO HAVE SEX WITH MEN WITH HIGH-RISK BEHAVIORS. Journal of Global Antimicrobial Resistance, In press, ⟨10.1016/j.jgar.2021.01.012⟩. ⟨hal-03134798⟩
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