micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation
Résumé
We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the
concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This
concentration module consists of a microfluidic channel with the shape of a funnel operated with
electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than one minute for
miR-21 alone, and then demonstrate that miR-21 levels measured in fine needle biopsy samples
from patients with pancreatic cancer correlate with the reference technique of reverse-
transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising
clinical performances for the follow-up of patients with cancer.
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